Índices de trombocitos en perros infectados con Ehrlichia canis y Key words: Anaplasma phagocytophilum, Ehrlichia canis,indices, thrombocyte (Source. Molecular survey of Ehrlichia canis in dogs from Mexico: prevalence of infection and possible associated factors. Estudio molecular de Ehrlichia canis en perros. Caracterizar filogenéticamente Ehrlichia canis a partir de perros naturalmente infectados y sus garrapatas, mediante PCR y secuenciación del.
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First phylogenetic analysis of Ehrlichia canis in dogs and ticks from Mexico. Facultad de Medicina Veterinaria y Zootecnia. Phylogenetic characterization of Ehrlichia canis in dogs naturally infected and ticks, diagnosed by PCR and sequencing of 16SrRNA gene; compare different isolates found in American countries. Were collected Blood samples from dogs with suggestive clinical manifestations of this disease and they were infested with ticks; part of 16SrRNA gene was perross and aligned, with perrow sequences reported in American countries.
Two phylogenetic trees were constructed using the Maximum likelihood method, and Maximum parsimony. They were positive to E. The clinical manifestations presented were fever, fatigue, depression and vomiting.
Rhipicephalus sanguineus Dermacentor variabilis and Haemaphysalis leporis-palustris ticks were positive for E. Phylogenetic analysis showed that the sequences of dogs and ticks in Mexico form a third group diverging of sequences from South America and USA. This is the first phylogenetic analysis of E. This research lays the foundation for further study of genetic variability. Fueron positivos a E.
Ehrlichiosis (canine) – Wikipedia
Las garrapatas Rhipicephalus sanguineus, Dermacentor variabilis y Haemaphysalis leporis-palustris fueron positivas para E. Ehrlichia canis is an obligate intracellular peros, the causative pathogen of Canine monocytic ehrlichiosis CMEwhich is a potentially zoonotic vector-borne disease 12.
The vector is Rhipicephalus sanguineus ticks. CME distribution is closely related to the distribution of the vector 3. The clinical symptoms commonly seen with this disease in infected dogs are fever, vomiting, arthralgia, rash, and diarrhea 5. Diagnosis is based on visualization of the morula in monocyte and enzymatic-immunoassay 4 ; however, as molecular tools 16SrRNA gene detection by PCR and sequencing have been successfully used for diagnosis 67.
Because of the high seroprevalence of infection on dogs from Mexico, is necessary to determine the actual frequency using molecular methods, and knowing the genetic variability of E. According to pertos, the aim of this study was to determine the frequency of Ehrlichia canis in dogs naturally infected, as well erlichiq infesting ticks it, comparing the sequences identified in Mexico with those published in GenBank from American countries, and perform phylogenetic analysis looking for the relation between isolates.
Study areas and samples collection. The study was conducted in Northwestern of Mexico, state of Sinaloa. Samples were collected from July to August In erlichiw study, dogs captured by perrs pound in three cities of North, Central and South, as well as dogs carried to any veterinary of Sinaloa were included.
As well as owners of sick dogs, workers of veterinary and kennels to participate in the study, prior informed consent; 88 owners and kennel workers accepted and we collected their blood samples for finding infection data not shown. Ticks were identified morphologically by entomologists, using dichotomous perrox. In each reaction was included a positive and negative distilled water control. Sequences were analyzed with Chromas program, and the search was conducted in GenBank database to determine the species and homology.
The phylogenetic trees were performed with the program MEGA v. The Neighbor-Join algorithm was used to obtain the initial tree, with 10 replicas of the sequences 10 Of dogs, were obtained 41 positive dogs with clinical manifestations Table 1. Were positive to Ehrlichia spp. The dogs risk to be infected with E. We sequenced six positive products for E.
KP showed a Two trees were performed: The tree was drawn to scale with branch lengths calculated using the average pathway method and are in the units of the number of changes over the whole sequence. The second tree was constructed by Maximum Parsimony method Figure 2.
The consistency index is 0. The Maximum parsimony tree was obtained using the Subtree-Pruning-Regrafting SPR algorithm with search level 0 in which the initial trees were obtained by the random addition of sequences 10 replicates.
These results confirm the existence of enzootic cycle transmission to E. This is the first report of this association in Mexico.
It was already experimentally demonstrated the competitiveness of this vector to transmit the bacteria 4. It is the first evidence of E. It may represent the presence of potential vectors, capable of transmitting E. More information is needed in order to prove if this vector can take on the role of competent vectors; ie, capable of transmitting the pathogen and causing the disease.
Although, Mexican isolates show differences between the groups I and II; the sequences found, presented an ancestral relationship with the sequences from Venezuela, Brazil and the United States; hypothesizing that it is possible to find the same variant of E.
It is required more phylogenetic analysis of E. This study presents the first molecular evidence of E. The results show a high risk of infection in tick-infested dogs. In Mexico, recent study in ticks proves a high frequency of infection of E. The importance of understanding the enzootic cycle of Ehrlichia in ticks, wild and domestic reservoirs, can help implement strategies to prevent infection in dogs 12 This research provides the assessment for further study of genetic variability in isolated not only from E.
The authors wish to thanks Abha Grover for grammar and language revision of this article. Guideline for veterinary prectitioners on canine Ehrlichiosis and anaplasmosis in Europe.
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Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molec Biol Evol ; Moler Biol Evol ; Tick-borne rickettsial pathogens in questing ticks, removing from humans and animals in Mexico. J Vet Sci ; 17 3: Med Clin N Am ; This is an open-access article distributed under the terms of the Creative Commons Attribution License.
Services on Demand Article. English pdf Article in xml format Article references How to cite this article Automatic translation Send this article by e-mail. Acknowledgments The authors wish to thanks Abha Grover for grammar and language revision of this article. How to cite this article.